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KMID : 0379119960240020155
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Korean Journal of Mycology
1996 Volume.24 No. 2 p.155 ~ p.165
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Phylogenetic Relationships Among Pleurotus species Inferred from Sequence Data of PCR Amplified ITS 2 Region in Ribosomal DNA
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이신우/Lee, Shin Woo
은무영/성기영/고승주/배신철/이인구/Eun, Moo Young/Seong, Ki Young/Go, Seung Joo/Bae, Shin Churl/Rhe, In Koo
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Abstract
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This study was carried out to identify the phylogenetic relationship among several isolates of Pleurotus species by comparing ITS II region of ribosomal DNA(rDNA) repeat unit. Two primers from ribosomal DNA sequences were chosen to amplify the specific internal transcribed spacer (ITS) II region of Pleurotus spp. The exact ITS II region with an unique band from six species of Pleurotus genus could be amplified using the two primers taken from at the 3´end of 5.8S rDNA and 5´-end of 285 rDNA. Six representative species of the Pleurotus genus were easily characterized according to the length differences of ITS II region. Furthermore, within P. ostreatus species, different sizes of ITS II region could be observed in the isolates of ASI 2025 and ASI 2095 although they were classified as P. oslrealus by the conventional observation. The nucleotide sequence analyses of PCR-amplified ITS II region indicated that the isolates ASI 2025 and ASI 2095 were different from other Pleurotus spp. When the nucleotide sequences of six Pleurotus species were compared, three typical ITS II regions were highly variable especially at both ends of this region. The phylogenetic tree obtained by the Neighbor program of Felsenstein PHILIP package with all the nucleotide sequence of Pleurotus spp. indicated that P. ostreatus, P. florida, P. sajor-caju and P. eryngii were closely related to one phylogenetic branch and P. cystidious was related to other branch with P. cornucopiae. The isolates ASI 2025 and 2038, however, were not closely related to any other Pleurotus spp. and formed their own individual branches.
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KEYWORD
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